dc.creator | Uribe, Pamela [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Excelencia en Medicina Traslacional] | |
dc.creator | Rojas, Christian [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Excelencia en Medicina Traslacional] | |
dc.creator | Merino, Juan [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Excelencia en Medicina Traslacional] | |
dc.creator | Zambrano, Fabiola [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Excelencia en Medicina Traslacional] | |
dc.creator | Villegas, JV. [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Biotecnología Reproductiva] | |
dc.creator | Boguen, Rodrigo [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Biotecnología Reproductiva] | |
dc.creator | Isachenko, Vladimir [Alemania. Universidad de Colonia. Grupo de Investigación en Medicina Reproductiva, Departamento de Obstetricia y Ginecología] | |
dc.creator | Isachenko, Evgenia [Alemania. Universidad de Colonia. Grupo de Investigación en Medicina Reproductiva, Departamento de Obstetricia y Ginecología] | |
dc.creator | Sánchez, Raúl [Chile. Núcleo de Biorecursos Científicos y Tecnológicos CEMT-BIOREN. Centro de Excelencia en Medicina Traslacional] | |
dc.creator | Treulen, Favian [Chile. Universidad Mayor. Facultad de Ciencias. Escuela de Tecnología Médica] | |
dc.date.accessioned | 2018-05-09T20:45:15Z | |
dc.date.available | 2018-05-09T20:45:15Z | |
dc.date.created | 2018-05-09T20:45:15Z | |
dc.date.issued | 2017-12 | |
dc.identifier | Uribe P, Rojas C, Meriño J, Zambrano F, Villegas J, Sánchez R, et al. Effect of incubation temperature after devitrification on quality parameters in human sperm cells. Cryobiology [serial on the Internet]. (2017, Dec), [cited May 9, 2018]; 7978-81. | |
dc.identifier | 0011-2240 | |
dc.identifier | eISSN: 1090-2392 | |
dc.identifier | WOS:000418107400012 | |
dc.identifier | ID de PubMed: 29037981 | |
dc.identifier | http://repositorio.umayor.cl/xmlui/handle/sibum/431 | |
dc.identifier | https://doi.org/10.1016/j.cryobiol.2017.10.005 | |
dc.identifier | 10.1016/j.cryobiol.2017.10.05 | |
dc.description.abstract | Sperm cryopreservation is common in assisted reproduction laboratories, providing a therapeutic option for several clinical conditions. This process has been optimized; however, the effect of post-thaw incubation temperature has been poorly studied. This work analyzed the effect of incubation temperature after devitrification on human sperm quality. Spermatozoa from normozoospermic donors were cryopreserved by vitrification. After devitrification, the spermatozoa were separated into two aliquots: (i) incubated at room temperature (RT, 22-25 °C) and (ii) incubated at 37 °C. Reactive oxygen species (ROS), viability, mitochondrial membrane potential (ΔΨM), phosphatidylserine externalization and motility were analyzed immediately after devitrification (control) and after 2, 4 and 6 h. Spermatozoa incubated at RT showed a conserved viability and ΔΨM compared to the control, while the incubation at 37 °C promoted a decrease in these parameters. The ROS levels were increased at both incubation conditions. The progressive motility was decreased in all experimental groups and the decrease was more pronounced under incubation at RT. No increase in phosphatidylserine externalization was observed. In conclusion, prior to use in assisted reproduction procedures, devitrified spermatozoa at RT conserve a better viability and ΔΨM than at 37 °C. | |
dc.language | en | |
dc.publisher | Chile. Universidad Mayor | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.title | Effect of incubation temperature after devitrification on quality parameters in human sperm cells | |
dc.type | Artículos de revistas | |