Artículos de revistas
Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: A randomized controlled trial
Date
2013-07-25Registration in:
Stem Cell Research and Therapy, v. 4, n. 4, 2013.
1757-6512
10.1186/scrt236
WOS:000323174400001
2-s2.0-84880335347
2-s2.0-84880335347.pdf
9643433706163946
6020984937849801
7773733250141398
Author
Universidade Estadual Paulista (UNESP)
Institutions
Abstract
Introduction. Tendon injury is a major cause of lameness and decreased performance in athletic equines. Various therapies for tendonitis have been described; however, none of these therapies results in complete tissue regeneration, and the injury recurrence rate is high even after long recovery periods involving rest and physiotherapy. Methods. A lesion was induced with collagenase gel in the superficial digital flexor tendon in the center portion of the metacarpal region of eight equines of mixed breed. After two weeks, the lesions of the animals in the treated and control groups were treated through the intralesional administration of mesenchymal stem cells derived from adipose tissue (adMSCs) suspended in platelet concentrate (PC) and with phosphate buffered saline (PBS), respectively. Serial ultrasound analyses were performed every two weeks. After 16 weeks of therapy, a biopsy was performed for histopathological, immunohistochemical and gene expression (type I collagen (COL1A1), type III collagen (COL3A1), tenascin-C (TNC), tenomodulin (TNMD), and scleraxis (SCX)) analyses. Results: Differences in the ultrasound and histopathological analyses were observed between the groups. Improved results were reported in the group treated with adMSCs suspended in PC. There was no difference in the gene expression levels observed after the different treatments. The main results observed from the histopathological evaluation of the treated group were as follows: a prevention of the progression of the lesion, a greater organization of collagen fibers, and a decreased inflammatory infiltrate. A lack of progression of the lesion area and its percentage was observed in the ultrasound image, and increased blood flow was measured by Power Doppler. Conclusions: The use of adMSCs combined with PC for the therapy of experimentally induced tendonitis prevented the progression of the tendon lesion, as observed in the ultrasound examination, and resulted in a greater organization and decreased inflammation, as observed in the histopathological evaluation. These data demonstrate the therapeutic potential of this therapy for the treatment of equine tendonitis. © 2013 Carvalho et al.; licensee BioMed Central Ltd.
Subjects
Related items
Showing items related by title, author, creator and subject.
-
Regional adaptations in three rat tendons
Covizi, DZ; Felisbino, SL; Gomes, L; Pimentel, ER; Carvalho, HF -
The effect of age on the structure and composition of rat tendon fibrocartilage
Esquisatto, MAM; Joazeiro, PP; Pimentel, ER; Gomes, L -
Ensayo mecánico de tracción-elongación para el ligamento cruzado craneal, tendón de aquiles, tendón patelar y ligamento sacrotuberoso en fresco y conservados en glicerina al 98% como posibles sustituto del ligamento cruzado craneal
García García, Olga Carolina; Reyes Rodríguez, Natalia Andrea