dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:22Z
dc.date.available2014-05-27T11:25:22Z
dc.date.created2014-05-27T11:25:22Z
dc.date.issued2010-12-01
dc.identifierGenetics and molecular research : GMR, v. 9, n. 4, p. 2317-2327, 2010.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/72110
dc.identifier10.4238/vol9-4gmr935
dc.identifier2-s2.0-79952197912
dc.identifier2-s2.0-79952197912.pdf
dc.description.abstractThe prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We analyzed three species of this genus (M. jelskii, M. amazonicum and M. brasiliense) using RAPD-PCR to assess their genetic variability, genetic structure and the phylogenetic relationship between them and to look for molecular markers that enable separation of M. jelskii and M. amazonicum, which are closely related syntopic species. Ten different random decamer primers were used for DNA amplification, yielding 182 fragments. Three of these fragments were monomorphic and exclusive to M. amazonicum or M. jelskii and can be used as specific molecular markers to identify and separate these two species. Similarity indices and a phylogenetic tree showed that M. amazonicum and M. jelskii are closest to each other, while M. brasiliense was the most differentiated species among them; this may be attributed to the different habitat conditions to which these species have been submitted. This information will be useful for further studies on these important crustacean species.
dc.languageeng
dc.relationGenetics and molecular research : GMR
dc.relation0,439
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectDNA
dc.subjectprimer DNA
dc.subjectagar gel electrophoresis
dc.subjectanimal
dc.subjectclassification
dc.subjectgenetic polymorphism
dc.subjectgenetics
dc.subjectnucleotide sequence
dc.subjectPalaemonidae
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectrandom amplified polymorphic DNA
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectDNA Primers
dc.subjectElectrophoresis, Agar Gel
dc.subjectPhylogeny
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Genetic
dc.subjectRandom Amplified Polymorphic DNA Technique
dc.titleGenetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR.
dc.typeArtículos de revistas


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