dc.creator | Contreras, Angela | |
dc.creator | Ribbeck, Magdalena | |
dc.creator | Gutiérrez, Guillermo D. | |
dc.creator | Cañon, Pablo M. | |
dc.creator | Mendoza, Sebastián N. | |
dc.creator | Agosin Trumper, Eduardo | |
dc.date.accessioned | 2019-03-18T12:01:21Z | |
dc.date.available | 2019-03-18T12:01:21Z | |
dc.date.created | 2019-03-18T12:01:21Z | |
dc.date.issued | 2018 | |
dc.identifier | Frontiers in Microbiology, Volumen 9, Issue MAR, 2018, | |
dc.identifier | 1664302X | |
dc.identifier | 10.3389/fmicb.2018.00291 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/167383 | |
dc.description.abstract | © 2018 Contreras, Ribbeck, Gutiérrez, Cañon, Mendoza and Agosin. The effect of ethanol on the metabolism of Oenococcus oeni, the bacterium responsible for the malolactic fermentation (MLF) of wine, is still scarcely understood. Here, we characterized the global metabolic response in O. oeni PSU-1 to increasing ethanol contents, ranging from 0 to 12% (v/v). We first optimized a wine-like, defined culture medium, MaxOeno, to allow sufficient bacterial growth to be able to quantitate different metabolites in batch cultures of O. oeni. Then, taking advantage of the recently reconstructed genome-scale metabolic model iSM454 for O. oeni PSU-1 and the resulting experimental data, we determined the redistribution of intracellular metabolic fluxes, under the different ethanol conditions. Four growth phases were clearly identified during the batch cultivation of O. oeni PSU-1 strain, according to the temporal consumption of malic and citric acids, sugar and amino acids uptake, and biosynthesis r | |
dc.language | en | |
dc.publisher | Frontiers Media S.A. | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Frontiers in Microbiology | |
dc.subject | Genome-scale metabolic model | |
dc.subject | Lactic acid bacteria | |
dc.subject | Malolactic fermentation | |
dc.subject | Oenococcus oeni | |
dc.subject | Physiological ethanol response | |
dc.subject | Wine-like defined culture medium | |
dc.title | Mapping the physiological response of Oenococcus oeni to ethanol stress using an extended genome-scale metabolic model | |
dc.type | Artículos de revistas | |