Artículo de revista
Sex hormone-binding globulin b expression in the rainbow trout ovary prior to sex differentiation
Fecha
2018Registro en:
General and Comparative Endocrinology, Volumen 259,
10956840
00166480
10.1016/j.ygcen.2017.11.021
Autor
Pérez, Claudio
Araneda, Cristian
Estay, Francisco
Díaz, Nelson F.
Vizziano-Cantonnet, Denise
Institución
Resumen
© 2017 Elsevier Inc.Salmonids have two sex hormone-binding globulin (Shbg) paralogs. Shbga is mainly expressed in the liver, while Shbgb is secreted by the granulosa cells of the rainbow trout ovary. Coexpression of shbgb and the gonadal aromatase cyp19a1a mRNAs been observed in granulosa cells, suggesting a physiological coordination between Shbgb expression and estrogen synthesis. As estrogens are essential for female sex determination in the fish ovary, we propose that Shbgb participates in early ovarian differentiation, either by binding with estrogen or through another mechanism that remains to be discovered. To elucidate this potential role, monosex populations of female trout were studied during the molecular ovarian differentiation period (28–56 dpf). shbgb mRNA expression was measured using qPCR and compared with expression of genes for other ovarian markers (cyp19a1a, foxl2, follistatin, and estrogen receptors). shbgb transcript expression was detected during the final stages