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Gold nanoparticles as an efficient drug delivery system for GLP-1 peptides
Fecha
2017Registro en:
Colloids and Surfaces B: Biointerfaces, Volumen 158,
18734367
09277765
10.1016/j.colsurfb.2017.06.015
Autor
Pérez Ortiz, Magdalena
Zapata Urzúa, Claudio Andrés
Acosta, Gerardo A.
Álvarez Lueje, Alejandro
Albericio, Fernando
Kogan Bocian, Marcelo
Institución
Resumen
© 2017 Elsevier B.V. In this work, the potential application of gold nanoparticles for GLP-1 analogues delivery was studied. For this purpose, the original sequence of the incretin GLP-1 was slightly modified in the C-terminal region by adding a cysteine residue to facilitate conjugation to the gold surface. The interaction between peptides and gold nanoparticles and also the colloid stability of the conjugates were studied by UV–vis spectrophotometry, TEM, IR and XPS spectroscopy. Moreover, the permeability of these conjugates was assayed using a Caco-2/goblet monolayer model. On the basis of the stability and permeability results, one of the conjugates was chosen to be administered intraperitoneally to normoglycemic rats. The intraperitoneal delivery of the GLP-1 analogue using gold nanoparticles led to decrease levels of blood glucose in the same way as native GLP-1, thereby demonstrating that the formulation of the analogue is stable in physiological conditions and maintains the ac