Artículo de revista
Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion
Date
2017Registration in:
Journal of Diabetes Research, Volumen 2017,
23146753
23146745
10.1155/2017/1328573
Author
Mizgier, María Luisa
Cataldo, Luis R.
Gutierrez, Juan
Santos, José Luis
Casas, Mariana
Llanos Vidal, Paola
Contreras Ferrat, Ariel Eduardo
Moro, Cedric
Bouzakri, Karim
Galgani Fuentes, José
Institutions
Abstract
© 2017 Maria L. Mizgier et al. Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes h