Artículo de revista
Trypanosoma cruzi induces cellular proliferation in the trophoblastic cell line BeWo
Fecha
2017Registro en:
Experimental Parasitology, Volumen 173,
10902449
00144894
10.1016/j.exppara.2016.12.005
Autor
Droguett, Daniel
Carrillo, Ileana
Castillo, Christian
Gómez, Fresia
Negrete, Miguel
Liempi, Ana
Muñoz, Lorena
Kemmerling Weis, Ulrike
Galanti Garrone, Norbel
Maya Arango, Juan
Droguett, Daniel
Institución
Resumen
© 2016 Elsevier Inc.Congenital transmission of Trypanosoma cruzi (T. cruzi) is partially responsible for the progressive globalization of Chagas disease. During congenital transmission the parasite must cross the placental barrier where the trophoblast, a continuous renewing epithelium, is the first tissue in contact with the parasite. The trophoblast turnover implies cellular proliferation, differentiation and apoptotic cell death. The epithelial turnover is considered part of innate immunity. We previously demonstrated that T. cruzi induces cellular differentiation and apoptosis in this tissue. Here we demonstrate that T. cruzi induces cellular proliferation in a trophoblastic cell line. We analyzed the cellular proliferation in BeWo cells by determining DNA synthesis by BrdU incorporation assays, mitotic index, cell cycle analysis by flow cytometry, as well as quantification of nucleolus organizer regions by histochemistry and expression of the proliferation markers PCNA and Ki67 by