Artículos de revistas
Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway
Fecha
2017Registro en:
Biochimica et Biophysica Acta - Molecular Cell Research, Volumen 1864, Issue 2, 2018, Pages 243-254
18792596
01674889
10.1016/j.bbamcr.2016.11.006
Autor
Maldonado, H.
Calderon, C.
Burgos Bravo, Francesca
Kobler, Oliver
Zuschratter, W.
Ramirez, O.
Härtel, S.
Schneider, P.
Quest, Andrew F. G.
Herrera-Molina, R.
Leyton, L.
Institución
Resumen
© 2016 Elsevier B.V. Two key proteins for cellular communication between astrocytes and neurons are αvβ3 integrin and the receptor Thy-1. Binding of these molecules in the same (cis) or on adjacent (trans) cellular membranes induces Thy-1 clustering, triggering actin cytoskeleton remodeling. Molecular events that could explain how the Thy-1-αvβ3 integrin interaction signals have only been studied separately in different cell types, and the detailed transcellular communication and signal transduction pathways involved in neuronal cytoskeleton remodeling remain unresolved. Using biochemical and genetic approaches, single-molecule tracking, and high-resolution nanoscopy, we provide evidence that upon binding to αvβ3 integrin, Thy-1 mobility decreased while Thy-1 nanocluster size increased. This occurred concomitantly with inactivation and exclusion of the non-receptor tyrosine kinase Src from the Thy-1/C-terminal Src kinase (Csk)-binding protein (CBP)/Csk complex. The Src inactivation dec