Artículo de revista
Immature rat ovaries become revascularized rapidly after autotransplantation and show a gonadotropin-dependent increase in angiogenic factor gene expression
Endocrinology, Volumen 134, Issue 3, 2018, Pages 1146-1154
Dissen, G. A.
Lara Peñaloza, Hernán
Fahrenbach, W. H.
Costa, M. E.
Ojeda, S. R.
When the ovaries of 23-day-old juvenile rats are transplanted to an ectopic site, they recover within 1 week the ability to control gonadotropin secretion via steroid negative feedback. Vascular corrosion casting followed by scanning electron microscopy revealed that the transplanted ovary becomes profusely revascularized within 48 h after transplantation. Vascular ingrowth was accompanied by a 40– to 60–fold increase in expression of the genes encoding two angiogenic factors, vascular endothelial growth factor (VEGF) and transforming growth factor-²1 (TGF ² 1), as assessed by RNA blot hybridization of the corresponding mRNAs. Although TGF ² 3 mRNA levels also increased, no changes in the levels of mRNAs encoding other putative angiogenic factors, such as TGF ±, basic fibroblast growth factor, and TGF ² 2, were observed. Hybridization histochemistry demonstrated that in intact ovaries, VEGF mRNA is mainly expressed in granulosa cells of the cumulus oophorus and thecal cells of large an