dc.creatorBriceño, Andrea
dc.creatorMuñoz, Patricia
dc.creatorBrito, Patricia
dc.creatorHuenchuguala, Sandro
dc.creatorSegura Aguilar, Juan
dc.creatorParis Pizarro, Irmgard
dc.date.accessioned2019-03-18T11:54:22Z
dc.date.available2019-03-18T11:54:22Z
dc.date.created2019-03-18T11:54:22Z
dc.date.issued2016
dc.identifierNeurotoxicity Research, Volumen 29, Issue 3, 2018, Pages 381-393
dc.identifier14763524
dc.identifier10298428
dc.identifier10.1007/s12640-015-9560-x
dc.identifierhttps://repositorio.uchile.cl/handle/2250/166815
dc.description.abstract© 2015, Springer Science+Business Media New York.In this study, we investigated the role of adducts formation between aminochrome and tubulin and its interference in microtubules assembly and stability in aminochrome-induced toxicity in SH-SY5Y cells. We also investigated whether changes in the microtubules structures are an early event that could affect tubulin expression. We demonstrated in vitro that aminochrome tubulin adducts inhibit tubulin polymerization and that aminochrome induces microtubules disassembly. Moreover, when the SH-SY5Y cells were incubated with aminochrome, we observed an increase in soluble tubulin, indicating depolymerization of microtubules. Aminochrome generates disruption of the microtubules network, leading to changes in the morphology of the cells inducing cell death, in a dose- and time-dependent manner. Interestingly, these changes preceded cell death and were partly inhibited by paclitaxel, a microtubule-stabilizing agent. Furthermore, we observed that
dc.languageen
dc.publisherSpringer New York LLC
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceNeurotoxicity Research
dc.subjectAminochrome
dc.subjectDopamine
dc.subjectMicrotubules polymerization
dc.subjectNeurodegeneration
dc.subjectParkinson’s disease
dc.subjectTubulin
dc.titleAminochrome Toxicity is Mediated by Inhibition of Microtubules Polymerization Through the Formation of Adducts with Tubulin
dc.typeArtículo de revista


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