dc.creatorCastillo, Juan P.
dc.creatorSánchez-Rodríguez, Jorge E.
dc.creatorHyde, H. Clark
dc.creatorZaelzer, Cristian A.
dc.creatorAguayo, Daniel
dc.creatorSepúlveda, Romina V.
dc.creatorLuk, Louis Y.P.
dc.creatorKent, Stephen B.H.
dc.creatorGonzález Nilo, Fernando
dc.creatorBezanilla, Francisco
dc.creatorLatorre, Ramón
dc.date.accessioned2019-03-18T11:54:20Z
dc.date.available2019-03-18T11:54:20Z
dc.date.created2019-03-18T11:54:20Z
dc.date.issued2016
dc.identifierProceedings of the National Academy of Sciences of the United States of America, Volumen 113, Issue 23, 2018, Pages E3231-E3239
dc.identifier10916490
dc.identifier00278424
dc.identifier10.1073/pnas.1606381113
dc.identifierhttps://repositorio.uchile.cl/handle/2250/166798
dc.description.abstractLarge-conductance Ca2+- and voltage-activated K+ (BK) channels are involved in a large variety of physiological processes. Regulatory β-subunits are one of the mechanisms responsible for creating BK channel diversity fundamental to the adequate function of many tissues. However, little is known about the structure of its voltage sensor domain. Here, we present the external architectural details of BK channels using lanthanide-based resonance energy transfer (LRET). We used a genetically encoded lanthanide-binding tag (LBT) to bind terbium as a LRET donor and a fluorophore-labeled iberiotoxin as the LRET acceptor for measurements of distances within the BK channel structure in a living cell. By introducing LBTs in the extracellular region of the α- or β1-subunit, we determined (i) a basic extracellular map of the BK channel, (ii) β1-subunit-induced rearrangements of the voltage sensor in α-subunits, and (iii) the relative position of the β1-subunit within the α/β1-subunit complex.
dc.languageen
dc.publisherNational Academy of Sciences
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceProceedings of the National Academy of Sciences of the United States of America
dc.subjectBK channels
dc.subjectLanthanide resonance energy transfer
dc.subjectβ 1-subunit
dc.titleβ1-subunit-induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel
dc.typeArtículo de revista


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