Artículos de revistas
Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration
Fecha
2016Registro en:
Biomaterials, Volumen 75,
18785905
01429612
10.1016/j.biomaterials.2015.10.014
Autor
Chávez, Myra Noemi
Schenck, Thilo Ludwig
Hopfner, Ursula
Centeno-Cerdas, Carolina
Somlai-Schweiger, Ian
Schwarz, Christian
Machens, Hans Günther
Heikenwalder, Mathias
Bono Merino, María Rosa
Allende Connelly, Miguel
Nickelsen, Jörg
Egaña, José Tomás
Institución
Resumen
© 2015 Elsevier Ltd. The use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues during dermal regeneration. In this work, we demonstrate the safety and efficacy of photosynthetic biomaterials in vivo after engraftment in a fully immunocompetent mouse skin defect model. Further, we show that it is also possible to genetically engineer such photosynthetic scaffolds to deliver other key molecules in addition to oxygen. As a proof-of-concept, biomaterials were loaded with gene modified microalgae expressing the angiogenic recombinant protein VEGF. Survival of the algae, growth factor delivery and regenerative potential were evaluated in vitro and in vivo. This work proposes the use of photosynthetic gene therapy in regenerative medicine and provides scientific evidence for t