dc.creator | Campos Acuña, Javier | |
dc.creator | Pérez, Francisco | |
dc.creator | Narváez, Edgar | |
dc.creator | Campos Mora, Mauricio | |
dc.creator | Gajardo, Tania | |
dc.creator | Catalán Martina, Diego | |
dc.creator | Aguillón Gutiérrez, Juan Carlos | |
dc.creator | Pino Lagos, Karina | |
dc.date.accessioned | 2019-03-18T11:52:17Z | |
dc.date.available | 2019-03-18T11:52:17Z | |
dc.date.created | 2019-03-18T11:52:17Z | |
dc.date.issued | 2015 | |
dc.identifier | Immunotherapy (2015) 7(2), 101–110 | |
dc.identifier | 17507448 | |
dc.identifier | 1750743X | |
dc.identifier | 10.2217/imt.14.116 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/166480 | |
dc.description.abstract | Aim: To date, there is no human dendritic cell (DC) based therapy to prevent allograft
rejection in transplanted patients. Here, we evaluate a potential protocol using a
murine in vivo transplant model. Materials & methods: We generated murine bone
marrow-derived DCs (BM-DCs), modulated with rapamycin (Rapa) and activated with
monophosphoryl lipid A (Rapamycin-treated and monophosphoryl lipid A-matured
DCs [Rapa-mDCs]). DCs phenotype was evaluated by flow cytometry, cytokine
production by ELISA and their T-cell stimulatory ability was tested in co-cultures
with CD4+ T cells. Using an in vivo skin graft model, we evaluated DCs tolerogenicity.
Results: In vitro, Rapa-mDCs exhibit a semi-mature phenotype given by intermediate
levels of co-stimulatory molecules and cytokines, and inhibit CD4+ T-cell proliferation.
In vivo, skin-grafted mice treated with Rapa-mDCs show high allograft survival,
accumulation of Foxp3+Tregs and cytokine pattern modification. Conclusion: RapamDCs re-educate the inflammatory microenvironment, promoting skin-allograft
survival. | |
dc.language | en | |
dc.publisher | Future Medicine | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Immunotherapy | |
dc.subject | Cellular therapy | |
dc.subject | Dendritic cells | |
dc.subject | Regulatory T cells | |
dc.subject | Tolerance | |
dc.title | Rapamycin-conditioned dendritic cells activated with monophosphoryl lipid-A promote allograft acceptance in vivo | |
dc.type | Artículo de revista | |