dc.creator | Alvarado Díaz, Carlos Patricio | |
dc.creator | Núñez González, Marco | |
dc.creator | Devoto, Luigi | |
dc.creator | González Ramos, Reinaldo | |
dc.date.accessioned | 2019-03-15T16:08:55Z | |
dc.date.available | 2019-03-15T16:08:55Z | |
dc.date.created | 2019-03-15T16:08:55Z | |
dc.date.issued | 2015 | |
dc.identifier | Fertility and Sterility Vol. 103, No. 2, February 2015 | |
dc.identifier | 15565653 | |
dc.identifier | 00150282 | |
dc.identifier | 10.1016/j.fertnstert.2014.10.046 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/166370 | |
dc.description.abstract | Objective: To evaluate the effect of iron overload on nuclear factor kappa-B (NF-kappa B) activation in human endometrial stromal cells (ESCs).
Design: Experimental study.
Setting: University hospital research laboratory.
Patient(s): Ten healthy women.
Intervention(s): Isolated ESCs from endometrial biopsies were incubated with 50 mu M FeSO4 or vehicle. The NF-kappa B inhibitor [5-(p-fluorophenyl)-2-ureido] thiophene-3-carboxamide (TPCA-1), which inhibits IKK beta, the kinase of I kappa B alpha (inhibitory protein of NF-kappa B), was used to prevent iron overload-stimulated NP-kappa B changes in ESCs.
Main Outcome Measure(s): NF-kappa B activation was assessed by p65:DNA-binding activity immunodetection assay. I kappa B alpha, p65, and intercellular adhesion molecule (ICAM)-1 proteins expression was evaluated by Western blots. ESC soluble ICAM (sICAM)-1 secretion was measured by ELISA using conditioned medium.
Result(s): Iron overload increased p65:DNA-binding activity and decreased I kappa B alpha and p65 cytoplasmic expression in ESCs after 30 minutes of incubation as compared with the basal condition. ESC ICAM-1 expression and sICAM-1 secretion were higher after 24 hours of iron overload treatment than in the absence of treatment. TPCA-1 prevented the iron overload-induced increase of p65:DNA binding and I kappa B alpha degradation.
Conclusion(s): Iron overload activates IKK beta in ESCs, stimulating the NF-kappa B pathway and increasing ICAM-1 expression and sICAM-1 secretion. These results suggest that iron overload induces a proendometriotic phenotype on healthy ESCs, which could participate in endometriosis pathogenesis and development. | |
dc.language | en | |
dc.publisher | Elsevier | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Fertility and Sterility | |
dc.subject | Endometriosis | |
dc.subject | endometrium | |
dc.subject | ICAM-1 | |
dc.subject | iron overload | |
dc.subject | NF-kB | |
dc.title | Iron overload-modulated nuclear factor kappa-B activation in human endometrial stromal cells as a mechanism postulated in endometriosis pathogenesis | |
dc.type | Artículo de revista | |