dc.creator | Bustamante, Mario | |
dc.creator | Fernández Verdejo, Rodrigo | |
dc.creator | Jaimovich Pérez, Enrique | |
dc.creator | Buvinic, Sonja | |
dc.date.accessioned | 2019-03-15T16:06:35Z | |
dc.date.available | 2019-03-15T16:06:35Z | |
dc.date.created | 2019-03-15T16:06:35Z | |
dc.date.issued | 2014 | |
dc.identifier | American Journal of Physiology - Endocrinology and Metabolism, Volumen 306, Issue 8, 2018, | |
dc.identifier | 15221555 | |
dc.identifier | 01931849 | |
dc.identifier | 10.1152/ajpendo.00450.2013 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/166159 | |
dc.description.abstract | Interleukin-6 (IL-6) is an important myokine that is highly expressed in skeletal muscle cells upon exercise. We assessed IL-6 expression in response to electrical stimulation (ES) or extracellular ATP as a known mediator of the excitation-transcription mechanism in skeletal muscle. We examined whether the canonical signaling cascade downstream of IL-6 (IL-6/JAK2/STAT3) also responds to muscle cell excitation, concluding that IL-6 influences its own expression through a positive loop. Either ES or exogenous ATP (100 μM) increased both IL-6 expression and p-STAT3 levels in rat myotubes, a process inhibited by 100 μM suramin and 2 U/ml apyrase. ATP also evoked IL-6 expression in both isolated skeletal fibers and extracts derived from whole FDB muscles. ATP increased IL-6 release up to 10-fold. STAT3 activation evoked by ATP was abolished by the JAK2 inhibitor HBC. Blockade of secreted IL-6 with a neutralizing antibody or preincubation with the STAT3 inhibitor VIII reduced STAT3 activatio | |
dc.language | en | |
dc.publisher | American Physiological Society | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | American Journal of Physiology - Endocrinology and Metabolism | |
dc.subject | Exercise | |
dc.subject | Muscle plasticity | |
dc.subject | Myokines | |
dc.subject | Purinergic signaling | |
dc.subject | Signal transducer and activator of transcription 3 | |
dc.title | Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca2+ signals and an IL-6 autocrine loop | |
dc.type | Artículos de revistas | |