Artículos de revistas
Diagnostic microbiologic methods in the GEMS-1 case/control study
Fecha
2012Registro en:
Clinical Infectious Diseases, Volumen 55, Issue SUPPL. 4, 2018,
10584838
15376591
10.1093/cid/cis754
Autor
Panchalingam, Sandra
Antonio, Martin
Hossain, Anowar
Mandomando, Inacio
Ochieng, Ben
Oundo, Joseph
Ramamurthy, T.
Tamboura, Boubou
Zaidi, Anita K.M.
Petri, William
Houpt, Eric
Murray, Patrick
Prado Jiménez, Valeria
Vidal, Roberto
Steele, Duncan
Strockbine, Nancy
Institución
Resumen
To understand the etiology of moderate-to-severe diarrhea among children in high mortality areas of sub-Saharan Africa and South Asia, we performed a comprehensive case/control study of children aged <5 years at 7 sites. Each site employed an identical case/control study design and each utilized a uniform comprehensive set of microbiological assays to identify the likely bacterial, viral and protozoal etiologies. The selected assays effected a balanced consideration of cost, robustness and performance, and all assays were performed at the study sites. Identification of bacterial pathogens employed streamlined conventional bacteriologic biochemical and serological algorithms. Diarrheagenic Escherichia coli were identified by application of a multiplex polymerase chain reaction assay for enterotoxigenic, enteroaggregative, and enteropathogenic E. coli. Rotavirus, adenovirus, Entamoeba histolytica, Giardia enterica, and Cryptosporidium species were detected by commercially available enzym