dc.creatorRiquelme, Gloria
dc.creatorDe Gregorio, Nicole
dc.creatorVallejos, Catalina
dc.creatorBerrios, Macarena
dc.creatorMorales, Bárbara
dc.date.accessioned2019-03-11T13:19:33Z
dc.date.available2019-03-11T13:19:33Z
dc.date.created2019-03-11T13:19:33Z
dc.date.issued2012
dc.identifierJournal of Membrane Biology, Volumen 245, Issue 3, 2018, Pages 141-150
dc.identifier00222631
dc.identifier14321424
dc.identifier10.1007/s00232-012-9422-x
dc.identifierhttps://repositorio.uchile.cl/handle/2250/165654
dc.description.abstractPotassium channels play important physiological roles in human syncytiotrophoblasts (hSTBs) from placenta, an epithelium responsible for maternal-fetal exchange. Basal and apical plasma membranes differ in their lipid and protein composition, and the latter contains cholesterol-enriched microdomains. In placental tissue, the specific localization of potassium channels is unknown. Previously, we described two isolated subdomains from the apical membrane (MVM and LMVM) and their respective microdomains (lipid rafts). Here, we report on the distribution of K ir2.1, K v2.1, TASK-1, and TREK-1 in hSTB membranes and the lipid rafts that segregate them. Immunoblotting experiments showed that these channels are present mainly in the apical membrane from healthy hSTBs. Apical expression versus basal membrane was 84 and 16% for K ir2.1 and K v2.1, 60 and 30% for TREK-1, and 74 and 26% for TASK-1. Interestingly, K v2.1 showed differences between apical membrane subdomains: 26 ± 8% was located in
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceJournal of Membrane Biology
dc.subjectApical membrane
dc.subjectBasal membrane
dc.subjectLipid raft
dc.subjectPlacenta
dc.subjectPotassium channel
dc.titleDifferential expression of potassium channels in placentas from normal and pathological pregnancies: Targeting of the K ir 2.1 channel to lipid rafts
dc.typeArtículo de revista


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