Artículo de revista
Trypanosoma cruzi induces apoptosis in ex vivo infected human chorionic villi
Fecha
2011Registro en:
Placenta, Volumen 32, Issue 5, 2018, Pages 356-361
01434004
10.1016/j.placenta.2011.02.005
Autor
Duaso, J.
Rojo, G.
Jaña, F.
Galanti Garrone, Norbel
Cabrera, G.
Bosco, C.
López Muñoz, Rodrigo
Maya Arango, Juan
Ferreira, J.
Kemmerling Weis, Ulrike
Institución
Resumen
Chagas' disease, produced by the haemoflagellated protozoan Trypanosoma cruzi (T. cruzi), is one of the most frequent endemic diseases in Latin America. In spite that in the past few years T. cruzi congenital transmission has become of epidemiological importance, studies about this mechanism of infection are scarce. The placental tissue undergoes apoptosis throughout gestation, as part of its normal turnover. On the other hand, it is known that T. cruzi induces, delays or inhibits apoptosis in other mammalian tissues. In order to determine the effect of parasite invasion on normal apoptosis in the placenta, explants of human chorionic villi were incubated with 105 trypomastigotes for 24 h. Effective infection was tested by visualizing T. cruzi antigens in histological preparations and by PCR. Upon infection, apoptotic cell death was determined by light and transmission electron microscopy, TUNEL analysis, measurement of caspase-3 like activity and immunohistochemical detection of caspa