Artículos de revistas
Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens
Fecha
2010Registro en:
Biological Research, Volumen 43, Issue 3, 2018, Pages 287-289
07176287
07169760
10.4067/S0716-97602010000300004
Autor
Toledo, Víctor
Ramírez, Galia
Valck, Carolina
López, Nandy
Ribeiro, Carolina H.
Maldonado, Ismael
Aguilar, Lorena
Lemus, David
Ferreira, Arturo
Institución
Resumen
Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the fact that the observed TcCRT effects can be attributed to the parasite-derived molecule and not to the endotoxin. The antiangiogenic TcCRT effects correlate with its anti-tumor in vivo effects, as recently shown in our laboratory.