dc.creator | Letelier, María Eugenia | |
dc.creator | Sánchez-Jofré, Sebastián | |
dc.creator | Peredo-Silva, Liliana | |
dc.creator | Cortés-Troncoso, Juan | |
dc.creator | Aracena-Parks, Paula | |
dc.date.accessioned | 2019-03-11T13:00:12Z | |
dc.date.available | 2019-03-11T13:00:12Z | |
dc.date.created | 2019-03-11T13:00:12Z | |
dc.date.issued | 2010 | |
dc.identifier | Chemico-Biological Interactions, Volumen 188, Issue 1, 2018, Pages 220-227 | |
dc.identifier | 00092797 | |
dc.identifier | 10.1016/j.cbi.2010.06.013 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/165062 | |
dc.description.abstract | Iron and copper ions, in their unbound form, may lead to the generation of reactive oxygen species via Haber-Weiss and/or Fenton reactions. In addition, it has been shown that copper ions can irreversibly and non-specifically bind to thiol groups in proteins. This non-specific binding property has not been fully addressed for iron ions. Thus, the present study compares both the pro-oxidant and the non-specific binding properties of Fe3+ and Cu2+, using rat liver cytosol and microsomes as biological systems. Our data show that, in the absence of proteins, Cu2+/ascorbate elicited more oxygen consumption than Fe3+/ascorbate under identical conditions. Presence of cytosolic and microsomal protein, however, differentially altered oxygen consumption patterns. In addition, Cu2+/ascorbate increased microsomal lipid peroxidation and decreased cytosolic and microsomal content of thiol groups more efficiently than Fe3+/ascorbate. Finally, Fe3+/ascorbate and Cu2+/ascorbate inhibited in different w | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Chemico-Biological Interactions | |
dc.subject | Catechin | |
dc.subject | Copper | |
dc.subject | Iron | |
dc.subject | Lipid peroxidation | |
dc.subject | N-Acetylcysteine | |
dc.subject | Thiol proteins | |
dc.title | Mechanisms underlying iron and copper ions toxicity in biological systems: Pro-oxidant activity and protein-binding effects | |
dc.type | Artículo de revista | |