dc.creator | Smith, P.C. | |
dc.creator | Guerrero, J. | |
dc.creator | Tobar, N. | |
dc.creator | Cáceres, M. | |
dc.creator | González, M. J. | |
dc.creator | Martínez, J. | |
dc.date.accessioned | 2019-03-11T12:56:48Z | |
dc.date.available | 2019-03-11T12:56:48Z | |
dc.date.created | 2019-03-11T12:56:48Z | |
dc.date.issued | 2009 | |
dc.identifier | Journal of Periodontal Research, Volumen 44, Issue 1, 2018, Pages 73-80 | |
dc.identifier | 00223484 | |
dc.identifier | 10.1111/j.1600-0765.2007.01081.x | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/164668 | |
dc.description.abstract | Background and Objectives: Membrane type 1-matrix metalloproteinase (MT1-MMP) is a collagenolytic enzyme involved in connective tissue remodeling. In periodontal tissues, either cytokines or growth factors regulate the production of proteolytic enzymes. Mice deficient in epidermal growth factor receptor (EGFR) show a reduced expression of MT1-MMP, suggesting that this receptor may play an important role in MT1-MMP production. The present study evaluated the role of the inflammatory cytokine tumor necrosis factor-α (TNF-α) and EGFR in the production of MT1-MMP in gingival fibroblasts. Material and Methods: Primary cultures of human gingival fibroblasts were cultured over plastic or a type I collagen matrix and stimulated with TNF-α and EGF. A selective EGFR inhibitor (AG1478) was used to interfere with this signaling pathway. Production of MT1-MMP and activation of proMMP-2 were studied using Western blot and gelatin zymography, respectively. Activation of EGFR signaling was assessed th | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Journal of Periodontal Research | |
dc.subject | Epidermal growth factor receptor | |
dc.subject | Fibroblast | |
dc.subject | Membrane type 1-matrix metalloproteinase | |
dc.subject | Tumor necrosis factor-α | |
dc.title | Tumor necrosis factor-α-stimulated membrane type 1-matrix metalloproteinase production is modulated by epidermal growth factor receptor signaling in human gingival fibroblasts | |
dc.type | Artículo de revista | |