Artículo de revista
First finding of Chagas disease vectors associated with wild bushes in the Metropolitan Region of Chile Primer hallazgo de vectores de la enfermedad de Chagas asociados a matorrales silvestres en la Región Metropolitana, Chile
Revista Medica de Chile, Volumen 134, Issue 10, 2018, Pages 1230-1236
García C., Alejandro
Javier Hidalgo, C.
Galuppo Gaete, Stephania
Cattan Ayala, Pedro
Background: Insects of the subfamily triatominae are the biological vectors of Trypanosoma cruzi, the causal agent of Chagas disease. Aim: To search for wild colonies of triatomines in the Metropolitan Region of Chile. Material and Methods: Ad hoc traps were placed in two endemic zones of the Metropolitan Region of Chile, during 30 nights. The dejections of 16 T infestans and 43 M spinolai specimens were examined under the microscope, searching for live metacyclic trypomastigotes. A polymerase chain reaction (PCR) was performed in macerates of all insects looking for T cruzi DNA. Results: A total of 269 bugs were captured. Forty four were Triatoma infestans and 225 were Mepraia spinolai. They were not syntopic, since T infestans was restricted to a Southern zone (Calera de Tango) while M spinolai was only found in the Northern zone (Til-Til). Both species were found associated to terrestrial bromeliads (Puya sp) but M spinolai was also detected in stony grounds. Microscopic examination