dc.creator | Mancilla, Alejandra | |
dc.creator | Cruz Vargas, Victor Ignacio | |
dc.creator | Izquierdo, Luis | |
dc.date.accessioned | 2019-01-29T17:57:08Z | |
dc.date.available | 2019-01-29T17:57:08Z | |
dc.date.created | 2019-01-29T17:57:08Z | |
dc.date.issued | 1997 | |
dc.identifier | Biocell, Volumen 21, Issue 3, 2018, Pages 167-174 | |
dc.identifier | 03279545 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/163939 | |
dc.description.abstract | Differentiation of the nonadherent trophectoderm cells of the mammalian embryo into attachment-competent trophoblast cells appears to be a necessary event for invasion of the uterine stroma. We have used a hanging drop culture system to investigate the involvement of contact with a solid surface and extracellular matrix proteins on mouse trophoblast differentiation. We report that laminin, but not fibronectin, promotes in vitro outgrowth of mouse trophoblast without attachment of the embryo to a solid surface, in a serum free culture medium. This result indicates that, in the presence of a trophoblast differentiation factor, the contact of the embryo with a solid surface is not necessary to obtain in vitro trophoblast differentiation. | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Biocell | |
dc.subject | Blastocyst | |
dc.subject | Laminin | |
dc.subject | Mouse embryo | |
dc.subject | Trophoblast | |
dc.title | Laminin promotes in vitro outgrowth of mouse trophoblast from blastocysts cultured in hanging drop | |
dc.type | Artículos de revistas | |