Artículos de revistas
Effect of associated bacteria on the growth and toxicity of Alexandrium catenella
Fecha
2003Registro en:
Applied and Environmental Microbiology, Volumen 69, Issue 1, 2018, Pages 659-662
00992240
10.1128/AEM.69.1.659-662.2003
Autor
Uribe, Paulina
Espejo Torres, Romilio
Institución
Resumen
Saprophytic bacteria in cultures of the marine dinoflagellate Alexandrium catenella were removed to assess their effect on growth and paralytic shellfish poisoning toxin production of this dinoflagellate. The actual axenic status was demonstrated by the lack of observable bacteria both immediately after treatment and following extended incubation in the absence of antibiotics. Bacteria were measured by counting CFU and also by epifluorescence microscopy and PCR amplification of bacterial 16S-23S spacer ribosomal DNA to detect noncultivable bacteria. Removal of bacteria did not have any effect on the growth of the dinoflagellate except for the inhibition of A. catenella disintegration after reaching the stationary phase. Toxicity was determined in dinoflagellate cell extracts by different methods: high-performance liquid chromatography (HPLC); an electrophysiological test called the Electrotest, which measures the inhibition of saxitoxin-sensitive Na+ channels expressed in a cell line;