dc.creatorHidalgo Tapia, María Cecilia
dc.creatorAracena, Paula
dc.creatorSánchez, Gina
dc.creatorDonoso Laurent, Paulina
dc.date.accessioned2019-01-29T17:51:09Z
dc.date.available2019-01-29T17:51:09Z
dc.date.created2019-01-29T17:51:09Z
dc.date.issued2002
dc.identifierBiological Research, Volumen 35, Issue 2, 2018, Pages 183-193
dc.identifier07169760
dc.identifier10.4067/S0716-97602002000200009
dc.identifierhttps://repositorio.uchile.cl/handle/2250/163521
dc.description.abstractIn skeletal and cardiac muscle cells, specific isoforms of the Ryanodine receptor channels mediate Ca2+ release from the sarcoplasmic reticulum. These channels are highly susceptible to redox modifications, which regulate channel activity. In this work, we studied the effects of Ca2+ (endogenous agonist) and Mg2+ (endogenous inhibitor) on the kinetics of Ca2+ release from sarcoplasmic reticulum vesicles isolated from skeletal or cardiac mammalian muscle. Native skeletal vesicles exhibited maximal stimulation of release kinetics by 10-20 μM [Ca2+], whereas in native cardiac vesicles, maximal stimulation of release required only 1 μM [Ca2+]. In 10 μM [Ca2+], free [Mg2+] < 0.1 mM produced marked inhibition of release from skeletal vesicles but free [Mg2+] ≤ 0.8 mM did not affect release from cardiac vesicles. Incubation of skeletal or cardiac vesicles with the oxidant thimerosal increased their susceptibility to stimulation by Ca2+ and decreased the inhibitory effect of Mg2+ in skeletal v
dc.languageen
dc.publisherSociety of Biology of Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceBiological Research
dc.subjectCalcium release kinetics
dc.subjectRedox state
dc.subjectRyanodine receptors
dc.subjectS-nitrosoglutathione
dc.subjectSarcoplasmic reticulum
dc.titleRedox regulation of calcium release in skeletal and cardiac muscle
dc.typeArtículos de revistas


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