dc.creator | Hidalgo Tapia, María Cecilia | |
dc.creator | Aracena, Paula | |
dc.creator | Sánchez, Gina | |
dc.creator | Donoso Laurent, Paulina | |
dc.date.accessioned | 2019-01-29T17:51:09Z | |
dc.date.available | 2019-01-29T17:51:09Z | |
dc.date.created | 2019-01-29T17:51:09Z | |
dc.date.issued | 2002 | |
dc.identifier | Biological Research, Volumen 35, Issue 2, 2018, Pages 183-193 | |
dc.identifier | 07169760 | |
dc.identifier | 10.4067/S0716-97602002000200009 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/163521 | |
dc.description.abstract | In skeletal and cardiac muscle cells, specific isoforms of the Ryanodine receptor channels mediate Ca2+ release from the sarcoplasmic reticulum. These channels are highly susceptible to redox modifications, which regulate channel activity. In this work, we studied the effects of Ca2+ (endogenous agonist) and Mg2+ (endogenous inhibitor) on the kinetics of Ca2+ release from sarcoplasmic reticulum vesicles isolated from skeletal or cardiac mammalian muscle. Native skeletal vesicles exhibited maximal stimulation of release kinetics by 10-20 μM [Ca2+], whereas in native cardiac vesicles, maximal stimulation of release required only 1 μM [Ca2+]. In 10 μM [Ca2+], free [Mg2+] < 0.1 mM produced marked inhibition of release from skeletal vesicles but free [Mg2+] ≤ 0.8 mM did not affect release from cardiac vesicles. Incubation of skeletal or cardiac vesicles with the oxidant thimerosal increased their susceptibility to stimulation by Ca2+ and decreased the inhibitory effect of Mg2+ in skeletal v | |
dc.language | en | |
dc.publisher | Society of Biology of Chile | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Biological Research | |
dc.subject | Calcium release kinetics | |
dc.subject | Redox state | |
dc.subject | Ryanodine receptors | |
dc.subject | S-nitrosoglutathione | |
dc.subject | Sarcoplasmic reticulum | |
dc.title | Redox regulation of calcium release in skeletal and cardiac muscle | |
dc.type | Artículos de revistas | |