dc.creatorFérez, Claudio F.
dc.creatorMarengo, Juan José
dc.creatorBull Simpfendorfer, Ricardo
dc.creatorHidalgo Tapia, María Cecilia
dc.date.accessioned2019-01-29T17:15:58Z
dc.date.available2019-01-29T17:15:58Z
dc.date.created2019-01-29T17:15:58Z
dc.date.issued1998
dc.identifierAmerican Journal of Physiology - Cell Physiology, Volumen 274, Issue 2 43-2, 2018,
dc.identifier03636143
dc.identifierhttp://repositorio.uchile.cl/handle/2250/163368
dc.description.abstractAdenosine 5'-cyclic diphosphoribose [cyclic ADP-ribose (cADPR)], a metabolite of NAD+ that promotes Ca2+ release from sea urchin egg homogenates and microsomal fractions, has been proposed to act as an endogenous agonist of Ca2+ release in sea urchin eggs. We describe experiments showing that a microsomal fraction isolated from Tetrapigus nyger sea urchin eggs displayed Ca2+-selective single channels with conductances of 155.0 ± 8.0 pS in asymmetric Cs+ solutions and 47.5 ± 1.1 pS in asymmetric Ca2+ solutions. These channels were sensitive to stimulation by Ca2+, ATP, and caffeine, but not inositol 1,4,5-trisphosphate, and were inhibited by ruthenium red. The channels were also activated by cADP-ribose in a Ca2+-dependent fashion. Calmodulin and Mg2+, but not heparin, modulated channel activity in the presence of cADP-ribose. We propose that these Ca2+ channels constitute the intracellular Ca2+-induced Ca2+ release pathway that is activated by cADP-ribose in sea urchin eggs.
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceAmerican Journal of Physiology - Cell Physiology
dc.subjectCalcium release channels
dc.subjectCalmodulin
dc.subjectEndoplasmic reticulum
dc.subjectIntracellular calcium
dc.subjectIntracellular channels
dc.subjectOocytes
dc.subjectRyanodine receptors
dc.titleCyclic ADP-ribose activates caffeine-sensitive calcium channels from sea urchin egg microsomes
dc.typeArtículos de revistas


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