dc.creator | Férez, Claudio F. | |
dc.creator | Marengo, Juan José | |
dc.creator | Bull Simpfendorfer, Ricardo | |
dc.creator | Hidalgo Tapia, María Cecilia | |
dc.date.accessioned | 2019-01-29T17:15:58Z | |
dc.date.available | 2019-01-29T17:15:58Z | |
dc.date.created | 2019-01-29T17:15:58Z | |
dc.date.issued | 1998 | |
dc.identifier | American Journal of Physiology - Cell Physiology, Volumen 274, Issue 2 43-2, 2018, | |
dc.identifier | 03636143 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/163368 | |
dc.description.abstract | Adenosine 5'-cyclic diphosphoribose [cyclic ADP-ribose (cADPR)], a metabolite of NAD+ that promotes Ca2+ release from sea urchin egg homogenates and microsomal fractions, has been proposed to act as an endogenous agonist of Ca2+ release in sea urchin eggs. We describe experiments showing that a microsomal fraction isolated from Tetrapigus nyger sea urchin eggs displayed Ca2+-selective single channels with conductances of 155.0 ± 8.0 pS in asymmetric Cs+ solutions and 47.5 ± 1.1 pS in asymmetric Ca2+ solutions. These channels were sensitive to stimulation by Ca2+, ATP, and caffeine, but not inositol 1,4,5-trisphosphate, and were inhibited by ruthenium red. The channels were also activated by cADP-ribose in a Ca2+-dependent fashion. Calmodulin and Mg2+, but not heparin, modulated channel activity in the presence of cADP-ribose. We propose that these Ca2+ channels constitute the intracellular Ca2+-induced Ca2+ release pathway that is activated by cADP-ribose in sea urchin eggs. | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | American Journal of Physiology - Cell Physiology | |
dc.subject | Calcium release channels | |
dc.subject | Calmodulin | |
dc.subject | Endoplasmic reticulum | |
dc.subject | Intracellular calcium | |
dc.subject | Intracellular channels | |
dc.subject | Oocytes | |
dc.subject | Ryanodine receptors | |
dc.title | Cyclic ADP-ribose activates caffeine-sensitive calcium channels from sea urchin egg microsomes | |
dc.type | Artículos de revistas | |