Artículos de revistas
Functional luteolysis in response to hydrogen peroxide in human luteal cells
Fecha
1995Registro en:
Journal of Endocrinology, Volumen 147, Issue 1, 2018, Pages 177-182
00220795
10.1677/joe.0.1470177
Autor
Vega Vega, Luis Felipe
Carrasco,
Castillo, Andrea
Troncoso, Sofía
Videla, Ximena
Devoto, Luigi
Institución
Resumen
To evaluate the effect of reactive oxygen species in human corpus luteum function, we investigated whether hydrogen peroxide (H2O2) affects the in vitro luteal cell production of steroids. H2O2 treatment (1.0-100 μM) of mid and late luteal cell cultures elicited a dose-dependent decrease in basal progesterone production. However, treatment of mid luteal cells with a low concentration of H2O2 (0.01 μM) significantly stimulated progesterone secretion (P < 0.05). In addition, H2O2 (100 μM) markedly inhibited human chorionic gonadotropin (hCG)-stimulated progesterone and estradiol secretion. cAMP production was enhanced (2.4-fold, P < 0.05) by hCG treatment of luteal cells. The addition of H2O2 (0.1-100 μM) to hCG-stimulated luteal cell cultures elicited a decrease in cAMP concentration (P < 0.05) and in the specific binding of radiolabeled hCG by luteal cells. Progesterone and estradiol production stimulated by dibutyryl cAMP were significantly inhibited by H2O2 (P < 0.05). These findings