dc.creatorCambiazo Ayala, Verónica
dc.creatorGonzález, Mauricio
dc.creatorMaccioni Baraona, Ricardo
dc.date.accessioned2019-01-29T14:55:15Z
dc.date.available2019-01-29T14:55:15Z
dc.date.created2019-01-29T14:55:15Z
dc.date.issued1995
dc.identifierJournal of Neurochemistry, Volumen 64, Issue 3, 2018, Pages 1288-1297
dc.identifier14714159
dc.identifier00223042
dc.identifier10.1046/j.1471-4159.1995.64031288.x
dc.identifierhttps://repositorio.uchile.cl/handle/2250/161318
dc.description.abstractAbstract: Microtubule‐associated proteins (MAPs) play major regulatory roles in the organization and integrity of the cytoskeletal network. Our main interest in this study was the identification and the analysis of structural and functional aspects of Drosophila melanogaster MAPs. A novel MAP with a relative molecular mass of 85 kDa from Drosophila larvae was found associated with taxol‐polymerized microtubules. In addition, this protein bound to mammalian tubulin in an overlay assay and coassembled with purified bovine brain tubulin in microtubule sedimentation experiments. The estimated stoichiometry of 85‐kDa protein versus tubulin in the polymers was 1:5.3 ± 0.2 mol/mol. It was shown that the 85‐kDa protein bound specifically to an affinity column of Sepharose‐βII‐(422–434) tubulin peptide, which contains the sequence of the MAP binding domain on βII‐tubulin. Affinity‐purified 85‐kDa protein enhanced microtubule assembly in a concentration‐dependent manner. This effect was signific
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceJournal of Neurochemistry
dc.subjectDrosophila melanogaster
dc.subjectDrosophila microtubule‐associated protein
dc.subjectMicrotubules
dc.subjectMicrotubule‐interacting proteins
dc.titleDMAP‐85: A τ‐Like Protein from Drosophila melanogaster Larvae
dc.typeArtículo de revista


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