dc.creator | Cambiazo Ayala, Verónica | |
dc.creator | González, Mauricio | |
dc.creator | Maccioni Baraona, Ricardo | |
dc.date.accessioned | 2019-01-29T14:55:15Z | |
dc.date.available | 2019-01-29T14:55:15Z | |
dc.date.created | 2019-01-29T14:55:15Z | |
dc.date.issued | 1995 | |
dc.identifier | Journal of Neurochemistry, Volumen 64, Issue 3, 2018, Pages 1288-1297 | |
dc.identifier | 14714159 | |
dc.identifier | 00223042 | |
dc.identifier | 10.1046/j.1471-4159.1995.64031288.x | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/161318 | |
dc.description.abstract | Abstract: Microtubule‐associated proteins (MAPs) play major regulatory roles in the organization and integrity of the cytoskeletal network. Our main interest in this study was the identification and the analysis of structural and functional aspects of Drosophila melanogaster MAPs. A novel MAP with a relative molecular mass of 85 kDa from Drosophila larvae was found associated with taxol‐polymerized microtubules. In addition, this protein bound to mammalian tubulin in an overlay assay and coassembled with purified bovine brain tubulin in microtubule sedimentation experiments. The estimated stoichiometry of 85‐kDa protein versus tubulin in the polymers was 1:5.3 ± 0.2 mol/mol. It was shown that the 85‐kDa protein bound specifically to an affinity column of Sepharose‐βII‐(422–434) tubulin peptide, which contains the sequence of the MAP binding domain on βII‐tubulin. Affinity‐purified 85‐kDa protein enhanced microtubule assembly in a concentration‐dependent manner. This effect was signific | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Journal of Neurochemistry | |
dc.subject | Drosophila melanogaster | |
dc.subject | Drosophila microtubule‐associated protein | |
dc.subject | Microtubules | |
dc.subject | Microtubule‐interacting proteins | |
dc.title | DMAP‐85: A τ‐Like Protein from Drosophila melanogaster Larvae | |
dc.type | Artículo de revista | |