Effect of zinc on human sperm motility and the acrosome reaction

Abstract

This study has assessed the effect of zinc on human sperm motility and the acrosome reaction in vitro. Progressively motile human sperm were selected by swim‐up and by glass bead columns and then incubated in a medium in which capacitation happened in an asynchronous way. Different doses of zinc (1, 10, 100 and 1000 μM) were added for periods of 2, 4 or 6 h. Other samples were incubated with zinc (1000 μM), and after 1 h incubation, the zinc was removed. Aliquots of each culture were used to evaluate progressive motility and the acrosome reaction using a triple‐stain technique. Sperm motility was reduced when the amount of zinc added was ≥ 100 μM, and these doses also caused a significant reduction in the % of sperm undergoing the acrosome reaction. After removal of zinc and further incubation in zinc‐free medium for 1 h, an increase in the percentage of motile and acrosome‐reacted sperm was observed. However, the increase in acrosome reaction did not reach the values observed in contr