Artículo de revista
Lipid composition of purified transverse tubule membranes isolated from amphibian skeletal muscle
Fecha
1987Registro en:
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Volumen 921, Issue 2, 2018, Pages 398-404
00052760
10.1016/0005-2760(87)90042-7
Autor
Pediconi, M. F.
Donoso P., Gonzalo
Hidalgo, C.
Barrantes, F. J.
Institución
Resumen
The level and proportion of lipids and their fatty acid composition were analyzed in highly purified transverse tubule membranes of amphibian skeletal muscle. Tubule membranes show (a) a higher content of lipids, (b) a higher phospholipid/cholesterol ratio and (c) a different phospholipid composition from other subcellular fractions, such as the light and heavy membranes from sarcoplasmic reticulum, which are similar in lipid profile. Transverse tubule membranes are characterized by a high percentage of phosphatidylserine and sphingomyelin and a low proportion of phosphatidylcholine compared with the other membranes. All three show a high proportion of ethanolamine plasmalogens (50% of the total ethanolamine glycerophospholipid). Transverse tubule membrane lipids contain a high proportion of 20- and 22-carbon polyunsaturated fatty acids, predominantly 20:4, 20:5, 22:5 and 22:6. Arachidonate predominates in phosphatidylinositol, eicosapentaenoate and docosahexaenoate in ethanolamine and