Pure platelet-rich plasma and supernatant of calcium-activated P-PRP induce different phenotypes of human macrophages

Abstract

Aim: This study aimed to evaluate the effect of two platelet preparations used in the clinic, pure platelet-rich plasma (P-PRP) and the supernatant of calcium-activated P-PRP (S-PRP), on the phenotype of human macrophages. Materials & methods: Surface markers and cytokine production of human monocyte-derived macrophages were analyzed after 24h stimulation with P-PRP or S-PRP. Results: P-PRP and S-PRP present no difference in the expression of CD206, a M2 tissue-repair macrophage-related marker. However, these same macrophages presented different levels of CD163, CD86 as well as different IL-10 secretion capacities after 24h incubation. Conclusion: These platelet preparations do not have an equivalent biological effect over macrophages, which suggest that they may present different clinical regenerative potentials.