dc.creator | Lefimil, Claudia | |
dc.creator | Lozano, Carla | |
dc.creator | Morales Bozo, Irene | |
dc.creator | Plaza, Anita | |
dc.creator | Maturana, Cristian | |
dc.creator | Urzúa Orellana, Blanca | |
dc.date.accessioned | 2018-12-20T15:22:38Z | |
dc.date.available | 2018-12-20T15:22:38Z | |
dc.date.created | 2018-12-20T15:22:38Z | |
dc.date.issued | 2013 | |
dc.identifier | Analytical Biochemistry, Volumen 433, Issue 2, 2013, Pages 129-131. | |
dc.identifier | 00032697 | |
dc.identifier | 10960309 | |
dc.identifier | 10.1016/j.ab.2012.10.024 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/158940 | |
dc.description.abstract | In the oral cavity, we can find a complex mixture of microorganisms, commensals, and pathogens. The studies of normal oral microbiota, as well as the studies of much oral pathology (e.g., caries, periodontitis), involve the isolation and cultivation of these microorganisms and their molecular analysis. The aim of this study was to validate a quick, easy, efficient, and inexpensive DNA extraction method for the recovery of genomic DNA from gram-positive and gram-negative oral bacteria to be used in polymerase chain reaction amplification. This method worked great with all samples analyzed, providing an approach to extract DNA for different microorganisms. | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Analytical Biochemistry | |
dc.subject | Aggregatibacter | |
dc.subject | DNA purification | |
dc.subject | Genomic DNA | |
dc.subject | Lactobacillus | |
dc.subject | Polymerase chain reaction | |
dc.subject | Porphyromonas | |
dc.subject | Streptococcus | |
dc.title | DNA from oral bacteria by sodium hydroxide-paper method suitable for polymerase chain reaction | |
dc.type | Artículos de revistas | |