dc.creatorLefimil, Claudia
dc.creatorLozano, Carla
dc.creatorMorales Bozo, Irene
dc.creatorPlaza, Anita
dc.creatorMaturana, Cristian
dc.creatorUrzúa Orellana, Blanca
dc.date.accessioned2018-12-20T15:22:38Z
dc.date.available2018-12-20T15:22:38Z
dc.date.created2018-12-20T15:22:38Z
dc.date.issued2013
dc.identifierAnalytical Biochemistry, Volumen 433, Issue 2, 2013, Pages 129-131.
dc.identifier00032697
dc.identifier10960309
dc.identifier10.1016/j.ab.2012.10.024
dc.identifierhttp://repositorio.uchile.cl/handle/2250/158940
dc.description.abstractIn the oral cavity, we can find a complex mixture of microorganisms, commensals, and pathogens. The studies of normal oral microbiota, as well as the studies of much oral pathology (e.g., caries, periodontitis), involve the isolation and cultivation of these microorganisms and their molecular analysis. The aim of this study was to validate a quick, easy, efficient, and inexpensive DNA extraction method for the recovery of genomic DNA from gram-positive and gram-negative oral bacteria to be used in polymerase chain reaction amplification. This method worked great with all samples analyzed, providing an approach to extract DNA for different microorganisms.
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceAnalytical Biochemistry
dc.subjectAggregatibacter
dc.subjectDNA purification
dc.subjectGenomic DNA
dc.subjectLactobacillus
dc.subjectPolymerase chain reaction
dc.subjectPorphyromonas
dc.subjectStreptococcus
dc.titleDNA from oral bacteria by sodium hydroxide-paper method suitable for polymerase chain reaction
dc.typeArtículos de revistas


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