Artículos de revistas
Cavβ2 transcription start site variants modulate calcium handling in newborn rat cardiomyocytes
Fecha
2015Registro en:
Pflugers Archiv European Journal of Physiology, Volumen 467, Issue 12, 2018, Pages 2473-2484
14322013
00316768
10.1007/s00424-015-1723-3
Autor
Moreno, Cristian
Hermosilla, Tamara
Morales, Danna
Encina, Matías
Torres-Díaz, Leandro
Díaz, Pablo
Sarmiento, Daniela
Simon, Felipe
Varela, Diego
Institución
Resumen
© 2015, Springer-Verlag Berlin Heidelberg.In the heart, the main pathway for calcium influx is mediated by L-type calcium channels, a multi-subunit complex composed of the pore-forming subunit CaV1.2 and the auxiliary subunits CaVα2δ1 and CaVβ2. To date, five distinct CaVβ2 transcriptional start site (TSS) variants (CaVβ2a-e) varying only in the composition and length of the N-terminal domain have been described, each of them granting distinct biophysical properties to the L-type current. However, the physiological role of these variants in Ca2+ handling in the native tissue has not been explored. Our results show that four of these variants are present in neonatal rat cardiomyocytes. The contribution of those CaVβ2 TSS variants on endogenous L-type current and Ca2+ handling was explored by adenoviral-mediated overexpression of each CaVβ2 variant in cultured newborn rat cardiomyocytes. As expected, all CaVβ2 TSS variants increased L-type current density and produced distinctive changes