Artículo de revista
Gold nanoparticles as tracking devices to shed light on the role of caveolin-1 in early stages of melanoma metastasis
Fecha
2018Registro en:
Nanomedicine, Volumen 13, Issue 12, 2018, Pages 1447-1462
17486963
17435889
10.2217/nnm-2017-0390
Autor
Guerrero, Simón
Díaz García, Víctor
Contreras Orellana, Pamela
Muñoz Lara, Pablo
Palma, Sujey
Guzmán, Fanny
Lobos González, Lorena
Cárdenas, Areli
Rojas Silva, Ximena
Muñoz, Luis
Leyton Campos, Lisette
Kogan Bocian, Marcelo
Quest, Andrew F. G.
Institución
Resumen
AIM
To track early events during lung metastasis, we labeled cells expressing (B16F10CAV1) or lacking CAV1 (B16F10mock) with gold nanoparticles conjugated to the peptide TAT (AuNPs-PEG-TAT).
METHODS
B16F10 expressing or lacking CAV1 were labeled with AuNPs-PEG-TAT. The physicochemical properties and cytotoxicity of these nanoparticles, as well as their effects on migration and invasiveness of B16F10 cells in vitro were evaluated. Ex vivo lung distribution of the labeled cells after tail vein injection into C57BL/6 mice was examined.
RESULTS
AuNPs-PEG-TAT did not affect B16F10 viability, migration and invasiveness. The metastatic and tumorigenic capability of the labeled B16F10 was also not modified in comparison to unlabeled B16F10 cells. CAV1 expression favored the retention of B16F10 cells in the lungs of mice 2 h post injection, suggesting CAV1 promoted adherence to endothelial cells and transendothelial migration.
CONCLUSIONS
We developed a protocol to label B16F10 cells with AuNPs-PEG-TAT that permits subsequent tracking of cells in mice. CAV1 overexpression was found to increase retention and transendothelial migration of B16F10 cells in the lung.