dc.creator | Parrado, Juan | |
dc.creator | Escuredo, Pedro R. | |
dc.creator | Conejero-Lara, Francisco | |
dc.creator | Kotik, Michael | |
dc.creator | Ponting, Christopher P. | |
dc.creator | Asenjo de Leuze, Juan | |
dc.creator | Dobson, Christopher M. | |
dc.date.accessioned | 2018-12-20T15:05:02Z | |
dc.date.available | 2018-12-20T15:05:02Z | |
dc.date.created | 2018-12-20T15:05:02Z | |
dc.date.issued | 1996 | |
dc.identifier | Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Volumen 1296, Issue 2, 2018, Pages 145-151 | |
dc.identifier | 01674838 | |
dc.identifier | 10.1016/0167-4838(96)00062-3 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/157633 | |
dc.description.abstract | Molecular characterisation of a lytic thermoactive β-1,3-glucanase from Oerskovia xanthineolytica LL-G109 has been performed. A molecular mass of 27 195.6 ± 1.3 Da and an isoelectric point of 4.85 were determined by electrospray mass spectrometry and from its titration curve, respectively. Its thermoactivity profile shows it to be a heat-stable enzyme with a temperature optimum of 65°C. The secondary structure content of the protein was estimated by circular dichroism to be approx. 25% α-helix, 7% random coil, and 68% β-sheet and β-turn structure. Nuclear magnetic resonance spectra confirm the high content of β-structure. Furthermore, the presence of a compact hydrophobic core is indicated by the presence of slowly exchanging amide hydrogens and the enzyme's relatively high resistance to proteolysis. The N-terminal sequences of the intact protein and of a tryptic peptide each exhibit significant similarity to family 16 of glycosyl hydrolases whose overall fold is known to contain almos | |
dc.language | en | |
dc.publisher | Elsevier B.V. | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology | |
dc.subject | Circular dichroism | |
dc.subject | Nuclear magnetic resonance | |
dc.subject | O. xanthineolytica | |
dc.subject | Thermoinactivation | |
dc.subject | β-1,3-Glucanase | |
dc.title | Molecular characterisation of a thermoactive β-1,3-glucanase from Oerskovia xanthineolytica | |
dc.type | Artículos de revistas | |