| dc.creator | Aguillón Gutiérrez, Juan Carlos | |
| dc.creator | Smith, P. | |
| dc.creator | Colombo, A. | |
| dc.creator | Molina, M. C. | |
| dc.creator | Ferreira, A. | |
| dc.date.accessioned | 2018-12-20T14:53:10Z | |
| dc.date.available | 2018-12-20T14:53:10Z | |
| dc.date.created | 2018-12-20T14:53:10Z | |
| dc.date.issued | 1997 | |
| dc.identifier | Arch. med. vet. v.29 n.1 Valdivia 1997 | |
| dc.identifier | 0301732X | |
| dc.identifier | https://repositorio.uchile.cl/handle/2250/157257 | |
| dc.description.abstract | Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Igs. This antibody was affinity purified in a Sepharose-protein G column. By immunoradiometric assay against the immunogen, the rabbit IgG anti-salmon Igs showed a high specific titer (at least 1/100.000) for the antigen. This reagent offers the possibility of detection of salmon or trout (crossreactivity) humoral immune responses, both for diagnostic and research purposes. | |
| dc.language | sp | |
| dc.source | Archivos de Medicina Veterinaria | |
| dc.subject | Diagnosis | |
| dc.subject | Polyclonal antibodies | |
| dc.subject | Salmon | |
| dc.title | Anticuerpos policlonales antiinmunoglobulinas
de salmón; herramientas potenciales para diagnóstico | |
| dc.type | Artículo de revista | |
