Evidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis

dc.creatorMuñoz, Sergio
dc.creatorVenegas Hermosilla, Juan
dc.creatorSolari Illescas, Aldo
dc.date.accessioned2018-12-20T14:52:43Z
dc.date.available2018-12-20T14:52:43Z
dc.date.created2018-12-20T14:52:43Z
dc.date.issued1991
dc.identifierInternational Journal for Parasitology, Volumen 21, Issue 7, 1991, Pages 863-866
dc.identifier00207519
dc.identifier10.1016/0020-7519(91)90156-2
dc.identifierhttps://repositorio.uchile.cl/handle/2250/157200
dc.description.abstractEvidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis. International Journal for Parasitology bd21: 863-866. Eight kinetoplast DNA samples from very different T. cruzi zymodemes were digested with the isoschizomer group of enzymes (MspI-HpaII) and (MboI-Sau 3AI), able to detect DNA methylation on cytidine and adenine for the CCGG and GATC sequences, respectively. Restriction digestion analysis of each kDNA with both isoschizomer groups of enzymes did not display a different profile suggesting that maxicircles and minicircles on this trypanosomatid are not methylated
dc.languageen
dc.sourceInternational Journal for Parasitology
dc.subject5-methyl cytidine
dc.subject6-methyl adenine
dc.subjectisoschizomers
dc.subjectKinetoplast DNA
dc.subjectT. cruzi
dc.titleEvidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis
dc.typeArtículo de revista


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