dc.creator | Lagos Mónaco, Rosalba | |
dc.creator | Wilkens, Marcela | |
dc.creator | Vergara Montecinos, Cecilia | |
dc.creator | Cecchi, Ximena | |
dc.creator | Monasterio Opazo, Octavio | |
dc.date.accessioned | 2018-12-20T14:34:22Z | |
dc.date.available | 2018-12-20T14:34:22Z | |
dc.date.created | 2018-12-20T14:34:22Z | |
dc.date.issued | 1993 | |
dc.identifier | FEBS Letters, Volumen 321, Issue 2-3, 2018, Pages 145-148 | |
dc.identifier | 00145793 | |
dc.identifier | 10.1016/0014-5793(93)80096-D | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/156518 | |
dc.description.abstract | Microcin E492, a polypeptide antibiotic, has been shown to have an Mr, of 6,000 by urea-SDS-polyacrylamide gel electrophoresis of the fluorescently labelled compound. It is known that the bactericidal action of microcin involves a loss of the transmembrane potential. In this study we show that microcin forms cation-selective channels in planar phospholipid bilayers. The channels have two main conductance states the current-voltage curves of which rectify. The reversal potentials measured under biionic conditions indicate a permeability sequence of NH4+ > K+ = Rb+ = Cs+ > Na+ = Li+ > Tris+. The results suggest that membrane potential dissipation induced by microcin is a consequence of the formation of pores in the bacterial membrane. © 1993. | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | FEBS Letters | |
dc.subject | Antibiotic | |
dc.subject | Ion channel | |
dc.subject | Klebsiella pneumoniae | |
dc.subject | Lipid bilayer | |
dc.subject | Microcin E492 | |
dc.title | Microcin E492 forms ion channels in phospholipid bilayer membranes | |
dc.type | Artículos de revistas | |