dc.creatorEncinas, M. Victoria
dc.creatorRojas, M. Cecilia
dc.creatorGoldie, Hughes
dc.creatorCardemil, Emilio
dc.date.accessioned2018-12-20T14:34:22Z
dc.date.available2018-12-20T14:34:22Z
dc.date.created2018-12-20T14:34:22Z
dc.date.issued1993
dc.identifierBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Volumen 1162, Issue 1-2, 2018, Pages 195-202
dc.identifier01674838
dc.identifier10.1016/0167-4838(93)90147-J
dc.identifierhttps://repositorio.uchile.cl/handle/2250/156517
dc.description.abstractTwo members of the ATP-dependent class of phosphoenolpyruvate (PEP) carboxykinases (Saccharomyces cerevisiae and Escherichia coli PEP carboxykinase), and one member of the GTP-dependent class (the cytosolic rat liver enzyme) have been comparatively analyzed by taking advantage of their intrinsic fluorescence. The S. cerevisiae and the rat liver enzymes show intrinsic fluorescence with a maximum emission characteristic of moderately buried tryptophan residues, while the E. coli carboxykinase shows somewhat more average exposure for these fluorophores. The fluorescence of the three proteins was similarly quenched by the polar compound acrylamide, but differences were observed for the ionic quencher iodide. For the ATP-dependent enzymes, these last experiments indicate more exposure to the aqueous media of the tryptophan population of the E. coli than of the S. cerevisiae enzyme. The effect of nucleotides on the emission intensities and quenching efficiencies revealed substrate-induced co
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
dc.subjectConformational change
dc.subjectNucleotide binding
dc.subjectPhosphoenol pyruvate carboxykinase
dc.subjectTryptophan fluorescence
dc.titleComparative steady-state fluorescence studies of cytosolic rat liver (GTP), Saccharomyces cerevisiae (ATP) and Escherichia coli (ATP) phosphoenolpyruvate carboxykinases
dc.typeArtículo de revista


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