dc.creator | Kessi, Eduardo | |
dc.creator | Guixé Leguía, Victoria Cristina | |
dc.creator | Preller, Ana | |
dc.creator | Ureta, Tito | |
dc.date.accessioned | 2018-12-20T14:32:12Z | |
dc.date.available | 2018-12-20T14:32:12Z | |
dc.date.created | 2018-12-20T14:32:12Z | |
dc.date.issued | 1996 | |
dc.identifier | Biochemical Journal, Volumen 315, Issue 2, 2018, Pages 455-460 | |
dc.identifier | 02646021 | |
dc.identifier | 10.1042/bj3150455 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/156309 | |
dc.description.abstract | Glycogen is the main end product of glucose metabolism in amphibian oocytes. However, in the first few minutes after [U-14C]glucose microinjection most of the label is found in lactate. The burst of lactate production and the shape of the time curves for the labelling of glucose 6-phosphate, fructose 6-phosphate, glucose 1-phosphate and glycogen suggest a precursor-product relationship of lactate with respect to glycogen and its intermediates. Expansion (by microinjection) of the pool of glycolytic intermediates, such as dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, 3-phosphoglycerate or phosphoenolpyruvate, results in a marked decrease in [U-14C]glucose incorporation into glycogen. After co-injection of doubly labelled glucoses, extensive detritiation (93%) of the glucosyl units of glycogen was observed with [2-3H,U-14C]glucose and partial detritiation with [3-3H,U-14C]glucose (34%) or [5-3H,U-14C]glucose (46%). After injection of [6-3H,U-14C]glucose, a small but significant | |
dc.language | en | |
dc.publisher | Portland Press Ltd | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Biochemical Journal | |
dc.subject | Biochemistry | |
dc.subject | Molecular Biology | |
dc.subject | Cell Biology | |
dc.title | Glycogen synthesis in amphibian oocytes: Evidence for an indirect pathway | |
dc.type | Artículos de revistas | |