Tolerance of native Magellan fungi in peat to Anthracene and n-dodecane for potential use in bioremediation Tolerancia a antraceno y n-dodecano de hongos nativos de turba Magallánica para su potencial uso en biorremediación

Abstract

© 2016, Pontificia Universidad Catolica de Chile, Facultad de Agronomia e Ingenieria Forestal. All rights reserved.The tolerances of filamentous Magellan fungi in peat were analyzed in the presence of structurally different petroleum hydrocarbons to assess their abilities for bioremediation in contaminated soils. Fungi on PDA (potato dextrose agar) and fresh peat plates were cultured and purified. The morphologically identified species were grown in a hydrocarbon adapted-fungi (HAF) medium. Increasing concentrations of anthracene, n-dodecane and furfural were applied to observe tolerances based on the radial growth kinetics of hyphae. The results showed that the radial growths of hyphae in anthracene reached average speeds of 10.95 ± 1.21, 11.03 ± 3.14 and 10.96 ± 4.61 mm h-1 in0.1, 1 and 2 g L-1 solutions, respectively. The average growth rates in n-dodecane were 10.52 ± 3.33, 14.67 ± 1.88 and 10.86 ± 3.50 mm h-1 in 10, 20 and 40 g L-1 solutions, respectively. The growth rate in furfu