dc.creator | Ortiz, Rocío | |
dc.creator | Antilén, Mónica | |
dc.creator | Speisky Cosoy, Hernán | |
dc.creator | Aliaga, Margarita E. | |
dc.creator | López Alarcón, Camilo | |
dc.date.accessioned | 2018-12-20T14:13:11Z | |
dc.date.available | 2018-12-20T14:13:11Z | |
dc.date.created | 2018-12-20T14:13:11Z | |
dc.date.issued | 2011 | |
dc.identifier | Journal of AOAC International, Volumen 94, Issue 5, 2018, Pages 1562-1566 | |
dc.identifier | 10603271 | |
dc.identifier | 10.5740/jaoacint.11-033 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/154898 | |
dc.description.abstract | The analytical parameters of the microplatebased oxygen radicals absorbance capacity (ORAC) method using pyrogallol red (PGR) as probe (ORAC-PGR) are presented. In addition, the antioxidant capacity of commercial beverages, such as wines, fruit juices, and iced teas, is estimated. A good linearity of the area under the curve (AUC) versus Trolox concentration plots was obtained [AUC = (845 ± 110) + (23 ± 2) [Trolox, μM], R = 0.9961, n = 19]. QC experiments showed better precision and accuracy at the highest Trolox concentration (40 μM) with RSD and REC (recuperation) values of 1.7 and 101.0%, respectively. When red wine was used as sample, the method also showed good linearity [AUC = (787 ± 77) + (690 ± 60) [red wine, μL/mL]; R = 0.9926, n = 17], precision and accuracy with RSD values from 1.4 to 8.3%, and REC values that ranged from 89.7 to 103.8%. Additivity assays using solutions containing gallic acid and Trolox (or red wine) showed an additive protection of PGR given by the samples | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Journal of AOAC International | |
dc.subject | Analytical Chemistry | |
dc.subject | Food Science | |
dc.subject | Environmental Chemistry | |
dc.subject | Agronomy and Crop Science | |
dc.subject | Pharmacology | |
dc.title | Analytical parameters of the microplate-based ORAC pyrogallol red assay | |
dc.type | Artículos de revistas | |