dc.creatorOróstica Arévalo, María Lorena
dc.creatorGarcía, Paula
dc.creatorVera, Carolina
dc.creatorGarcía, Victor
dc.creatorRomero Osses, Carmen
dc.creatorVega Blanco, María Margarita
dc.date.accessioned2018-11-07T20:54:10Z
dc.date.available2018-11-07T20:54:10Z
dc.date.created2018-11-07T20:54:10Z
dc.date.issued2018
dc.identifierReproductive Sciences 2018, Vol. 25(7) 1000-1009
dc.identifier10.1177/1933719117732157
dc.identifierhttps://repositorio.uchile.cl/handle/2250/152472
dc.description.abstractPolycystic ovary syndrome (PCOS) affects not only ovarian functions but is also able to affect endometrium metabolism. Around 80% of women with PCOS are obese. High tumor necrosis factor (TNF)- production and low adiponectin levels are characteristics of obesity. Interestingly, endometrium from obese women with PCOS presents an insulin-resistance condition, high TNF- levels, and low adiponectin levels. However, TNF- effect on molecules associated with insulin action in endometrial cells remains unclear. Therefore, the objective of this work was to evaluate TNF- effect on expression of molecules associated with adiponectin (insulin sensitizing) and TNF- signaling pathways and on Glucose Transporter type 4 (GLUT-4) levels in human endometrial cells under the characteristic conditions of hyperandrogenic/hyperinsulinic (HA/HI) PCOS. Two human endometrial stromal cell lines (T-HESC/St-T1b) under HA/HI conditions were used to assay the effect of high TNF- concentration (100 ng/mL) on adiponectin, AdipoR1-AdipoR2 receptors, Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), Phospho-AMP-activated protein kinase T172 (p-AMPKT172), GLUT-4, Tumor necrosis factor receptor 1 (TNFR1)-Tumor necrosis factor receptor 2 (TNFR2) receptors protein levels, and nuclear factor B (NFB) nuclear content, by Western blot or immunocytochemistry. The NFB participation in TNF- effect on adiponectin expression was assayed using an NFB inhibitor (pyrrolidine dithiocarbamate). The TNF- increases the expression of molecules associated with its own signaling pathway (P < .05) and decreases the protein levels of adiponectin and its associated molecules (P < .05). Moreover, TNF- increases NFB nuclear content (P < .001), whereas with NFB inhibition the decrease in adiponectin content induced by TNF- was not observed. GLUT-4 levels were lower with TNF- treatment (P < .01). Thus, in human endometrial stromal cells, high TNF- levels negatively affect the insulin action through decreased adiponectin signaling and GLUT-4 protein. This could explain the failures observed in endometrial function of obese women with PCOS.
dc.languageen
dc.publisherSage
dc.sourceReproductive Sciences
dc.subjectEndometrial cells
dc.subjectTNF-alpha
dc.subjectAdiponectin
dc.subjectInsulin
dc.subjectGLUT-4
dc.titleEffect of TNF-alpha on molecules related to the Iinsulin action in endometrial cells exposed to hyperandrogenic and hyperinsulinic conditions characteristics of polycystic ovary syndrome
dc.typeArtículo de revista


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