Chile
| Artículo de revista
Thyroid hormone induced expression of the hepatic scaffold proteins sestrin2, beta Klotho, and FRS2 in relation to FGF21 AMPK signaling
dc.creator | Videla Cabrera, Luis | |
dc.creator | Vargas, Romina | |
dc.creator | Riquelme, Barbara | |
dc.creator | Fernández, Javier | |
dc.creator | Fernández Arancibia, Virginia | |
dc.date.accessioned | 2018-08-07T20:11:22Z | |
dc.date.available | 2018-08-07T20:11:22Z | |
dc.date.created | 2018-08-07T20:11:22Z | |
dc.date.issued | 2018 | |
dc.identifier | Experimental and Clinical Endocrinology & Diabetes, 126 (3): 182-186, marzo 2018 | |
dc.identifier | 10.1055/s-0043-115533 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/150711 | |
dc.description.abstract | Thyroid hormone (3,3', 5-triiodothyronine, T-3) accelerates energy metabolism in the liver through mechanisms involving upregulation of AMP-activated protein kinase (AMPK). This study aims to assess the influence of T-3 on the expression of the scaffold proteins beta-Klotho, fibroblast growth factor receptor substrate 2 alpha (FRS2 alpha), and Sestrin2 in relation to FGF21-AMPK signaling. Male Sprague-Dawley rats were given 0.1 mg T-3/kg or hormone vehicle (controls) and studies were done 24 h after treatment. These include measurements of the mRNA expression (qPCR) of hepatic beta-Klotho, FGF21, FGF21 receptor-1 (FGFR1), extracellular-signal-regulated kinase 1/2 (ERK1/2), FRS2 alpha, ribosomal S6 kinase-1 (RSK1), liver kinase B1 (LKB1), AMPK, and Sestrin2. Also, protein levels of FGF21, FGFR1 (ELISA), and ERK1/2 (Western blot) were measured. T-3 elicited a calorigenic response with higher hepatic mRNA expression of beta-Klotho, FRS2 alpha, and FGF21, increased serum FGF21, without changes in liver FGFR1 mRNA and its plasma levels. In addition, T-3 enhanced ERK1/2 phosphorylation and the mRNA expression of ERK1/2, RSK1, LKB1, AMPK, and Sestrin2. T-3 administration enhances liver FGF21-AMPK signaling involving upregulation of the scaffold proteins beta-Klotho, FRS2 alpha, and Sestrin2. beta-Klotho and FRS2 induction favours the operation of the FGF21-FGFR1-beta-Klotho complex as evidenced by the enhancement in ERK1/2 phosphorylation, whereas that of Sestrin2 recruits LKB1 to achieved AMPK activation, thus supporting a higher energy expenditure condition that may be desirable in some metabolic disorders | |
dc.language | en | |
dc.publisher | Johann Ambrosius Barth Verlag Medizinverlage Heidelberg GMBH | |
dc.source | Experimental and Clinical Endocrinology & Diabetes | |
dc.subject | Thyroid hormone | |
dc.subject | Liver | |
dc.subject | Beta Klotho | |
dc.subject | Sestrin2 | |
dc.subject | FGF21 AMPK signaling | |
dc.title | Thyroid hormone induced expression of the hepatic scaffold proteins sestrin2, beta Klotho, and FRS2 in relation to FGF21 AMPK signaling | |
dc.type | Artículo de revista |