Artículo de revista
Exoenzymes and metabolites related to the nematicidal effect of rhizobacteria on Xiphinema index Thorne & Allen
Fecha
2016Registro en:
Journal of Applied Microbiology Volumen: 120 Número: 2 Páginas: 413-424 Feb 2016
DOI: 10.1111/jam.12987
Autor
Castañeda Álvarez, C.
Prodan, Simona
Rosales, I. M.
Aballay Espinoza, Erwin
Institución
Resumen
AimsTo identify enzymes and metabolites in the rhizobacteria filtrates that have a nematicidal effect
on Xiphinema index and perform molecular characterization of the strains evaluated.
Methods and ResultsA series of four bacteria selected for their nematicidal potential were
considered for invitro, biochemical and molecular studies. The direct effect of the bacterial filtrates
was evaluated invitro on X.index juveniles and adults. Hydrogen sulphide and hydrogen cyanide
liberation and protease, chitinase, collagenase and lipase activity were verified in the strains. Up to
five housekeeping genes and one ITS 16S-23S rRNA were analysed. All bacterial filtrates
presented 54-100% mortality when evaluated during up to 72h of nematode exposure. Strains
presented protease activity; two of them (strains FB833T and FR203A) showed reliable collagenase
and chitinase activities, respectively, and three of them showed strong lipolytic activity (FB833T,
FR203A and FS213P). Strain Bacillus megaterium FB133M had no lipase activity and presented
the lowest nematicidal effect. Bacillus amyloliquefaciens FR203A had the largest lethal effect.
ConclusionThe rhizobacteria strains evaluated in this study possess nematicidal compounds, which
may offer an interesting alternative for X.index control.
Significance and Impact of the StudyThis is the first report of exoenzymes and metabolites
associated with nematicidal effect of rhizobacteria on X.index, which can be a possible alternative
for control of this plant-parasitic nematode.