dc.creator | Mazaki-Tovi, Shali | |
dc.creator | Vaisbuch, Edi | |
dc.creator | Tarca, Adi L. | |
dc.creator | Kusanovic, Juan Pedro | |
dc.creator | Than, Nandor Gabor | |
dc.creator | Chaiworapongsa, Tinnakorn | |
dc.creator | Dong, Zhong | |
dc.creator | Hassan, Sonia S. | |
dc.creator | Romero, Roberto | |
dc.date.accessioned | 2016-01-09T02:45:29Z | |
dc.date.available | 2016-01-09T02:45:29Z | |
dc.date.created | 2016-01-09T02:45:29Z | |
dc.date.issued | 2015 | |
dc.identifier | PLoS ONE
10(12): e0143779, 2015 | |
dc.identifier | DOI: 10.1371/journal.pone.0143779 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/136298 | |
dc.description.abstract | Objective
The purpose of this study was to compare the transcriptome of visceral and subcutaneous adipose tissues between pregnant and non-pregnant women.
Study Design
The transcriptome of paired visceral and abdominal subcutaneous adipose tissues from pregnant women at term and matched non-pregnant women (n = 11) was profiled with the Affymetrix Human Exon 1.0 ST array. Differential expression of selected genes was validated with the use of quantitative reverse transcription-polymerase chain reaction.
Results
Six hundred forty-four transcripts from 633 known genes were differentially expressed (false discovery rate (FDR) <0.1; fold-change >1.5), while 42 exons from 36 genes showed differential usage (difference in FIRMA scores >2 and FDR<0.1) between the visceral and subcutaneous fat of pregnant women. Fifty-six known genes were differentially expressed between pregnant and non-pregnant subcutaneous fat and three genes in the visceral fat. Enriched biological processes in the subcutaneous adipose tissue of pregnant women were mostly related to inflammation.
Conclusion
The transcriptome of visceral and subcutaneous fat depots reveals pregnancy-related gene expression and splicing differences in both visceral and subcutaneous adipose tissue. Furthermore, for the first time, alternative splicing in adipose tissue has been associated with regional differences and human parturition. | |
dc.language | en | |
dc.publisher | Public Library Science | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | |
dc.subject | Gestational diabetes-mellitus | |
dc.subject | Tumor-necrosis-factor | |
dc.subject | Maternal serum adiponectin | |
dc.subject | Messenger-RNA expression | |
dc.subject | C-Reactive protein | |
dc.subject | Colony-enhancing factor | |
dc.subject | Glucose-transporter expression | |
dc.subject | Plasma Visfatin Concentrations | |
dc.subject | Retinol-binding protein-4 | |
dc.title | Characterization of Visceral and Subcutaneous Adipose Tissue Transcriptome and Biological Pathways in Pregnant and Non-Pregnant Women: Evidence for Pregnancy-Related Regional-Specific Differences in Adipose Tissue | |
dc.type | Artículo de revista | |