Artículo de revista
International Regulations of Propolis Quality: Required Assays do not Necessarily Reflect their Polyphenolic-Related In Vitro Activities
Fecha
2015Registro en:
Journal of Food Science Vol. 80, Nr. 6, 2015
DOI: 10.1111/1750-3841.12881
Autor
Bridi, Raquel
Montenegro, Gloria
Núñez Quijada, Gabriel
Giordano, Ady
Morán Romero, María Fernanda
Jara Pezoa, Isaac
Speisky Cosoy, Hernán
Atala, Elías
López Alarcón, Camilo
Institución
Resumen
Propolis has been proposed as a polyphenolic-rich natural product potentially able to be used for human consumption or even for medicinal proposes. To guarantee a minimum phenolic and flavonoid content and as consequence of their related-biological activities, international requirements of propolis quality are commonly applied. In this work we assessed phenolic and flavonoid contents of propolis; the antioxidant capacity (toward peroxyl radicals and hypochlorous acid); the ability to generate nitric oxide (NO); and, finally the antimicrobial activity of 6 propolis samples from the VI region of Chile. Our results show that the total phenolic and flavonoid content of propolis samples are not always in agreement with their polyphenolic-associated in vitro activities. For example, P03 and P06 samples showed the lowest (25 4 GAE/g propolis) and the highest (105 +/- 3 GAE/g propolis) total phenolic content, respectively. This was in agreement with flavonoid content and their Oxygen Radical Absorbance Capacity (ORAC) activity. However, this dependence was not observed toward HOCl, NO release and antimicrobial activity. Based on our results, we consider that, in order to guarantee the antioxidant or antimicrobial in vitro effects, the international regulations of propolis quality should contemplate the convenience of incorporating other simple analytical test such as ORAC or antimicrobial tests.
Practical Application Together with the titration of polyphenols and flavonoids, it is necessary to apply other assays to guarantee antioxidant capacity (as in the ORAC method) and antimicrobial activity of propolis samples. These assays should be considered by authorities for including in the international regulations of propolis quality.