Artículo de revista
Production of Cell-Penetrating Peptides in Escherichia coli Using an Intein-Mediated System
Fecha
2015Registro en:
Appl Biochem Biotechnol (2015) 175:3025–3037
0273-2289
DOI: 10.1007/s12010-015-1484-7
Autor
Rodríguez Gallardo, Vida
Lascani Monsalve, Jorge
Asenjo de Leuze, Juan
Andrews Farrow, Bárbara
Institución
Resumen
Cell-penetrating peptides are molecules with the ability to cross membranes and
enter cells. Attention has been put on these peptides as a tool for drug delivery research, as they
are able to serve as delivery vectors for large molecules. Intracellular delivery of bioactive
peptides is a very promising research area for clinical applications, since peptides are able to
simulate protein regions and thus modulate key intracellular protein-protein interactions.
Therefore, evaluation of different strategies for production of these peptides is necessary. In
this work, an intein-mediated system was used to evaluate Escherichia coli recombinant
production of p53pAnt and PNC27 anticancer cell-penetrating peptides. It was demonstrated
that the pTXB1 and the pTYB11 vector systems are suitable for production of this kind of
peptides. The production process involves a low-temperature induction process and an efficient
on-column intein-mediated cleavage, which allowed an effective peptide recovery using
a single chromatographic step.