Artículos de revistas
Prenatal Stress Down-Regulates Reelin Expression by Methylation of Its Promoter and Induces Adult Behavioral Impairments in Rats
Fecha
2015Registro en:
PLOS ONE February 13, 2015 1 / 24
doi: 10.1371/journal.pone.0117680
Autor
Palacios García, Ismael
Lara Vásquez, Ariel
Montiel, Juan F.
Díaz Véliz, Gabriela
Sepúlveda, Hugo
Utreras Puratich, Elías
Montecino, Martín
González Billault, Christian
Aboitiz, Francisco
Institución
Resumen
Prenatal stress causes predisposition to cognitive and emotional disturbances and is a risk
factor towards the development of neuropsychiatric conditions like depression, bipolar disorders
and schizophrenia. The extracellular protein Reelin, expressed by Cajal-Retzius
cells during cortical development, plays critical roles on cortical lamination and synaptic
maturation, and its deregulation has been associated with maladaptive conditions. In the
present study, we address the effect of prenatal restraint stress (PNS) upon Reelin expression
and signaling in pregnant rats during the last 10 days of pregnancy. Animals from one
group, including control and PNS exposed fetuses, were sacrificed and analyzed using
immunohistochemical, biochemical, cell biology and molecular biology approaches. We
scored changes in the expression of Reelin, its signaling pathway and in the methylation of
its promoter. A second group included control and PNS exposed animals maintained until
young adulthood for behavioral studies.